Trace of holonomy around a fixed loop defines a function on the space of unitary connections on a hermitian vector bundle over a Riemannian manifold. Using the derivative of trace of holonomy, the loop, and a flat unitary connection, a functional is defined on the vector space of twisted degree 1 cohomology classes with coefficients in skew-hermitian bundle endomorphisms. It is shown that this functional is obtained by pairing elements of cohomology with a degree 1 homology class built directly from the loop and equipped with a flat section obtained from the variation of holonomy around the loop. When the base manifold is closed Kähler, hard Lefschetz duality implies that the space of twisted degree 1 cohomology classes is a symplectic vect...