Analgesic activity test of ethyl acetate, n-hexane, water fractions of cemcem leaves extract (Spondias pinnata (Linn.f.) Kurz.) as part of Usadha Bali

Pain is the most common reason why patients come to seek medical help. Pain is an unpleasant sensory and emotional feeling and is associated with tissue damage. Indonesia is one of the countries that still uses traditional medicine in addition to formal treatment. Spondias pinnata plant (Linn.f.) Kurz or Cemcem Plant is one that is used empirically as a pain reliever during menstrual pain by Balinese people. This study aims to prove the analgesic activity of the leaves of Cemcem (Spondias pinnata (Linn.f.) Kurz) that grow in Bali by making Cemcem leaves in 3 (three) fractions including ethyl acetate fraction, n-hexane fraction, and water fraction, so there is a comparison of analgesic activity of the three fractions. The three types of fractions were subjected to phytochemical screening to determine the class of secondary metabolite compounds present in Cemcem leaves extract. Analgesic activity test uses heat induction method with a temperature of 55oC in 20 mice divided into four groups, namely the negative control group was given tween 80, the positive control group was given mefenamic acid 100 mg / kg BW, the ethyl acetate fraction group, the fraction group n-Hexane, and water fraction of Cemcem leaves extract