Ras gene mutations in bladder tumors

DNK, izolovana iz 32 tumora bešike, analizirana je na prisustvo tačkastih mutacija H-ras gena za koji se zna da učestvuje u razvitku različitih malignih tumora čoveka. Primenom tehnike hibridizacije oligonuklotidnim probama na in vitro umnoženu tumorsku DNK, tačkaste mutacije na 12. i 13. kodonu H-ras gena detektovane su u 7 od 32 tumorska uzorka (22%). Ovako niska učestalost mutacija H-ras gena upućuje na zaključak da njihova aktivacija nije dominantni genetski događaj u razvitku tumora bešike, bar ne u većem delu te tumorske populacije.The DNA from thirty-two bladder cancers was screened for the presence of H-ras gene mutations, known to be involved in the pathogenesis of various human malignancies. Using the technique of oligonucleotide hybridization on previously in vitro amplified tumor DNA, H-ras mutations at codon 12 and 13 were detected in 7 out of 32 tumor specimens (22%). This low incidence of mutated ras genes suggests that their activation does not represent the crucial event in bladder tumor development, at least in the major part of tumor population