Modelling Nitrogen Uptake in Winter Oilseed Rape by Using Influx Kinetics of Nitrate Transport Systems

A mechanistic model was proposed in order to predict nitrogen uptake by a culture of oilseed rape (Brassica napus L.), using independently measured characteristics of plants growing in hydroponic or under field conditions. Uptake kinetics of the different components (Constitutive and Inducible) of the Low and High Affinity Transport Systems of nitrate (CLATS, ILATS, CHATS and IHATS, respectively) were determined by 15NO3- labelling in controlled conditions. The use of kinetic equations of transport systems and the experimental field data from the INRA-Châlons rape databank allowed to model NO3- uptake during the plant growth cycle. The study of different factors such as root temperature, day/night cycle and ontogenetic stages on NO3- uptake rate has been undertaken in order to improve the model prediction. Model outputs show that the high affinity transport system (HATS) accounted for about 90 % of total NO3- uptake (20 and 70 % for CHATS and IHATS without fertilization, respectively). The low affinity transport system (LATS) accounted for a minor proportion of total N uptake, and its activity was restricted to the early phase of the growth cycle. However, N autumnal fertilization increased the duration of its contribution (from 67 to 100 days) to total N uptake