Antibacterial activity of Lamiaceae and Apiaceae essential oils on bacterias and fungi

Ova studija predstavlja orginalno istraživanje iz oblasti fitohemije, biotehnologije i fitofarmakologije bazirano na veoma značajnoj i interesantnoj problematici vezanoj za primenu etarskih ulja samostalno ili inkapsuliranih u hitozanske čestice u savremenoj antimikrobnoj terapiji genitalnih infekcija , koja su do sada bila korišćena uglavnom u tradicionalnoj medicini za tretiranje bakterijskih i gljivičnih infekcija. Pojava rezistentnosti mikroorganizama na postojeće antibiotike povećava potrebu za pronalaskom novih terapeutskih agenasa sa antimikrobnim delovanjem u ginekologiji. Posebno mesto imaju etarska ulja aromatičnih biljaka, gde spadaju biljke familije Apiaceae (kim, korijander, morač) i familije Lamiaceae (žalfija, origano i timijan). Upotreba etarskih ulja u antimikrobnoj terapiji je naročito interesantna jer ne postoji dokazana rezistencija mikroorganizama na etarska ulja upravo zbog velikog broja različitih jedinjenja u njihovom sastavu koja i pojedinačno ispoljavaju snažno antimikrobno delovanje, ali je izrazita specifičnost njihovih sinergističkih efekata koji u stvari i sprečavaju nastanak otpornosti. Rezultati ispitivanja ove doktorske disertacije jasno ukazuju na značajnu antimikrobnu aktivnost etarskog ulja timijana i njegove inkapsulacije u najsavremenije farmaceutske oblike, tzv Drug Delivery Systems koji bi kontrolisanim otpuštanjem aktivne supstance omogućili stalno prisustvo antimikrobnog agensa u toku antimikrobne terapije. Aktivnost hitozanskih čestica u kiseloj sredini, širok antibakterijski spektar timijana,kao i bakteriostatsko dejstvo istog na laktobacile, ukazuju na mogućnost izrade i upoterbe vaginalnih antimikrobnih fitopreparata u terapijske i profilaktičke svrhe.This study presents original research in the field of phyto-chemistry, biotechnology and phyto-pharmacology based on a very important and interesting issues related to the application of essential oils alone or encapsulated in chitosan particles in modern antimicrobial therapy of genital infections, which have so far mainly been used in traditional medicine for the treatment of bacterial and fungal infections. The emergence of resistant microorganisms to existing antibiotics increases the need for the discovery of new therapeutic agents with antimicrobial activity in gynecology. A special place for essential oils of aromatic plants, which include plants of the family Apiaceae (cumin, coriander, fennel) and Lamiaceae (sage, oregano and thyme). The use of essential oils in antimicrobial therapy is especially because there is no proven resistance of microorganisms to essential oils because of the large number of different compounds in their composition which individually exhibit strong antimicrobial activity, but was pronounced specificity of their synergistic effects, in fact, prevent the emergence of resistance. Test results of this doctoral dissertation clearly indicate significant antimicrobial activity of essential oils of thyme and its encapsulation in the latest pharmaceutical forms, the so-called Drug Delivery Systems that controlled release of active substance are constant presence of antimicrobial agents during antimicrobial therapy. Activity chitosan particles in an acidic medium, a wide antibacterial spectrum of thyme, and the bacteriostatic effect of the same on the lactobacilli, suggest the possibility of development and use of artificial vaginal antimicrobial herbal remedies for therapeutic and prophylactic purposes