Fatty acids of serum phospholipids as biomarkers of nutritional status in mouflon population: influence of age and gender

Cilj istraživanja bila je optimizacija metode za utvrđivanje masnokiselinskoga sastava fosfolipida seruma. Istraživanje je provedeno na populaciji od 40 mufl ona podijeljenih prema dobi na tri skupine (mlađi od dvije godine, između dvije i tri godine i stariji od tri godine) te prema spolu. Nakon izolacije ukupni lipidi razdvojeni su primjenom tankoslojne kromatografi je na polarne (fosfolipidi) i neutralne. Nakon razdvajanja fosfolipidi su transesterifi cirani u oblik metilnih estera masnih kiselina i analizirani metodom plinske kromatografi je. Dobiveni rezultati pokazali su značajan utjecaj dobi na koncentraciju pojedinih masnih kiselina i zbirni profi l. Nasuprot tomu, spol nije značajnije utjecao na koncentraciju masnih kiselina. Rezultati su potvrdili kako je riječ o primjenjivoj i relativno jednostavnoj metodi. Takva metoda može imati široki raspon primjene, od procjene hranidbenoga statusa i okoliša divljih životinja do kontrole i prevencije prekomjerne tjelesne mase, metaboličkoga sindroma i šećerne bolesti kod kućnih ljubimaca.The aim of the investigation was optimization of the method for determination of fatty acid composition of serum phospholipids. The study was conducted on a population of 40 moufl on divided into three age groups (under two years, between two and three years and older than three years) and into male and female group. After isolation, total lipids were separated using thin layer chromatography into polar (phospholipids) and neutral lipids. After the separation, phospholipids were transesterifeid and analyzed by gas chromatography in the form of fatty acid methyl esters. The results showed a signifi cant effect of age on the concentration of individual fatty acids and on the summarized profi le. In contrast, gender did not infl uence the concentration of fatty acids. Results have confi rmed that described method is applicable and relatively simple for routine analyses. The method can have a wide range of applications, from the assessment of wildlife nutritional status to the control and prevention of obesity, metabolic syndrome and diabetes in pets