Neutralising antibodies for bovine herpesvirus 1 and bovine viral diarrhoea virus in clinically infected cows

U ovome istraživanju pretraženo je 50 uzoraka seruma mliječnih krava podrijetlom s farme s ukupno 500 grla, a na kojoj se ne provodi cijepljenje protiv ZRG-a i VPG-a. Serumi su ciljano uzeti samo od krava koje su pokazivale kliničke znakove bolesti koji upućuju na infekciju s goveđim herpesvirusom 1 (GHV 1) i virusom virusnog proljeva goveda (VVPG). Serumski uzorci su pretraženi virus neutralizacijskim testom (VN-test) na prisutnost protutijela za navedene viruse. Na virus ZRG-a bilo je pozitivno 47 seruma te seroprevalencija za taj virus iznosi 94%. Na virus VPG/BSG-a bilo je pozitivno 49 seruma te seroprevalencija iznosi 98%. U velikom broju seruma ustanovljena su protutijela i za jedan i za drugi virus (92% uzoraka). Ustanovljena je povezanost vrste virusne infekcije s većinom kliničkih znakova, a naročito pojavnost ZRG-a s mastitisom i upalom pluća te VPG-a s mrtvorođenjem, kržljavošću, pobačajem, problemima s preživanjem i proljevom.This study tested a total of 50 cow serum samples from a single dairy farm with 1500 animals. Tested cows were not vaccinated but showed clinical signs indicating BHV-1 infection and/or BVDV infection. Serum samples were tested with the virus-neutralization test for the presence of neutralising antibodies for the two mentioned viruses. The presence of BHV-1 antibodies was observed in 47 serum samples, therefore the seroprevalence for this virus was 94%. Antibodies for BVDV were found in 49 serum samples and the seroprevalence was 98%. Antibodies for both viruses (BHV-1 and BVDV) were found in 92% of serum samples. Connections were found between the sort of viral infection and the presented clinical signs. There was a correlation between BHV-1 and the occurrence of mastitis and pneumonia. Correlations were also found between BVDV and stillbirth, stuntedness, abortion, rumination issues and diarrhoea