Review of the congruence between cytopathological and histopathological diagnoses of archived cases, from 2009 to 2016 at the Department of Veterinary Pathology, Zagreb

Citološka pretraga se rutinski primjenjuje u kliničkom radu radi svoje učinkovitosti, brzine i jednostavnosti. Patohistološka pretraga je složenija, zahtijeva više vremena za pripremu preparata, ali je ona zlatni standard za analiziranje arhitekture tkiva. Obje metode se provode na Zavodu za veterinarsku patologiju, no do sada nije ispitivana razina njihove podudarnosti za istovjetne lezije. Pretragom arhive Zavoda za veterinarsku patologiju, pronađeni su svi slučajevi (parovi) citoloških i histoloških pretraga od istovjetnih lezija u razdoblju od 2009. – 2016. godine, ukupno 251. Obavljena je statistička analiza ispravnosti i korisnosti citoloških slučajeva u odnosu na histološke. Ne uključujući nedostatne slučajeve (20,7%), 80,4% slučajeva pokazuje potpunu ili djelomičnu podudarnost, a 19,6% nepodudarnost. Većina uzoraka je bila upravo iz područja kože (50,6%), te je u tom području 20,5% dijagnoza činio mastocitom, dok su ostale dijagnoze činile 79,5%. Rezultati su pokazali 96,6% podudarnosti za mastocitom, neovisno o sustavu. Ovakvi rezultati su podjednaki onim dobivenima u sličnim istraživanjima, bilo u humanoj ili veterinarskoj medicini. Predmetno istraživanje je pokazalo visoku razinu podudarnosti između citopatoloških i histopatoloških dijagnoza te je dokazalo da je citopatološka pretraga, koja se nadopunjuje s histopatološkom pretragom, vrlo važna i korisna inicijalna i orijentacijska pretraga u klasificiranju kožnih lezija domaćih životinja.Cytological examination is routinely applied in clinical work because of its efficiency, speed and simplicity. Histological examination is more complex, andrequires more time for specimen preparation, but it is the gold standard for analysing tissue architecture. Both methods are performed by the Department of Veterinary Pathology of the Veterinary Faculty, University of Zagreb, but the rate of congruency between those methods has not yet been investigated. The Department archives were searched for all cases (pairs) that underwent both cytological and histological examination of the same lesion, between 2009 and 2016, that is, altogether 251 cases. Statistical analysis of the accuracy and efficiency of cytological cases in relation to histological cases was conducted. With indecisive (non-diagnostic) cases excluded (20.7%), the rate of complete or partial congruency between cytological and histological diagnosis was 80.4%, and the rate of non-congruency was 19.6%. The majority of samples were taken from the skin region (50.6%), where 20.5% were mastocytomas, in comparison to other lesions which altogether accounted for 79.5% of lesions. The results showed 96.6% congruency in mastocytoma cases, regardless of the region. The results are similar to those obtained in comparable research, in both human and veterinary medicine. This research showed a high rate of congruency between cytological and histological diagnosis and cytological examination was shown (supplemented by histological examination) to be a very important and useful screening method in the classification of skin lesions in domestic animals