Brzo odvajanje i određivanje flavonoida kapilarnom zonskom elektroforezom u uzorcima meda

Flavonoids have crucial applications in the biological and physiological fields. Honey, as an important sweet food made by bees, is rich in flavonoids. In this paper, the analytical method for flavonoids determination in different sorts of honey from different geographical locations was developed by capillary zone electrophoresis with direct ultraviolet detection. With a running buffer (borate, 20 mmol l–1) at pH of 8.4, four typical flavonoids, rutin, quercetin, kaempferol and myricetin, were separated in five minutes under a applied potential of 25 kV. A linear relationship within the range of 2.0 – 500 mg l–1 was found for these four kinds of flavonoids. Moreover, the detection limits ranged from 1.17 to 1.76 mg l–1. The recoveries lie in the range between 80 % – 107 %. The developed method was then used in the separation and determination of flavonoids in real honey samples collected from 12 geographical locations in the Henan Province of China. Rutin was detected in six, and quercetin in eight honey samples, which may be the markers for the identification of honey from different geographical origins. This work is licensed under a Creative Commons Attribution 4.0 International License.Flavonoidi nalaze važnu primjenu u biološkim i fiziološkim područjima. Med je važno slatko jelo bogato flavonoidima. U ovom radu razvijena je metoda za određivanje flavonoida u medu s različitih geografskih položaja kapilarnom zonskom elektroforezom uz UV detekciju. Četiri tipična flavonoida (rutin, kvercetin, kempferol, miricetin) odijeljena su za pet minuta u boratnom puferu (20 mmol l–1) pri pH = 8,4 uz primjenu potencijala 25 kV. Za ta četiri flavonoida linearna ovisnost vrijedila je u rasponu od 2,0 do 500 mg l–1. Granica detekcije je između 1,17 i 1,76 mg l–1. Iscrpak je u rasponu 80 – 107 %. Razvijena metoda upotrijebljena je za odvajanje i određivanje flavonoida u uzorcima meda s 12 mjesta u kineskoj provinciji Henan. Rutin je nađen u šest, a kvercetin u osam uzoraka pa bi ti flavonoidi mogli poslužiti za utvrđivanje geografskog porijekla meda. Ovo djelo je dano na korištenje pod licencom Creative Commons Imenovanje 4.0 međunarodna