Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System

Svrha: Usporediti antimikrobno djelovanje triju tehnika ispiranja korijenskog kanala s obzirom na jednako vrijeme ispiranja i jednak volumen sredstva za ispiranje. Ispitanici i metode: Četrdeset i osam jednokorijenskih zuba inokulirano je 48 sati suspenzijom Enterococcus faecalisa. Preostalih šest uzoraka korišteno je za negativnu kontrolu. Trideset i šest kanala nasumično je raspoređeno u tri eksperimentalne skupine: 1. grupa: konvencionalno ispiranje brizgalicom i iglom; 2. grupa: automatsko-dinamično ispiranje (RinsEndo); 3. grupa: pasivno ultrazvučno ispiranje (PUI). U prvom protokolu korišteno je 20 ml 3-postotnoga natrijeva hipoklorita, a u drugom protokolu kanali su isprani istim tehnikama i istom otopinom tijekom 45 sekundi. Uzorci iz korijenskih kanala kultivirani su, te su izbrojene bakterijske kolonije. Rezultati: U protokolu standardiziranog volumena irigansa, sistem RinsEndo bio je učinkovitiji od PUI-ja (p 0,05). U skupini RinsEndo pronađen je najveći broj uzoraka s minimalnim brojem bakterija E. faecalis. Zaključak: Pri ispiranju korijenskih kanala istim volumenom irigansa, sistem RinsEndo učinkovitiji je od PUI-ja. RinsEndom postignuto je i najveće smanjenje bakterija u oba protokola, koristeći se najmanjom količinom irigansa te ostvarujući najdulje vrijeme djelovanja irigansa u kanalu.Aim: The aim of the study was to compare the antimicrobial efficacy of three irrigation techniques after the use of standardized volume of NaOCl and with standardized time and irrigation. Methodology: Forty-eight single rooted teeth were inoculated with an Enterococcus faecalis suspension for 24 h. The remaining six canals served as negative controls. The 36 root canals were randomly distributed into three experimental groups; group 1, conventional syringe irrigation; group 2, automateddynamic irrigation (RinsEndo); group 3, passive ultrasonic irrigation (PUI). In the first protocol, the standardized volume of 3% NaOCl (20 mL) was used and in the second protocol, and standardized irrigation time (45 seconds) was used. Samples from root canals were cultured and the colony-forming units (CFUs) were counted. Results: When the volume of the irrigant was tandardized, RinsEndo was more effective than PUI (p0.05). The RinsEndo group had the highest percentage of minimal counts of E. faecalis CFUs. Conclusions: RinsEndo was more effective than PUI only when the volume of the irrigant was standardized. However, the RinsEndo provided higher bacterial reduction in both protocols when using the least amount of the irrigant and providing longer contact time