Determination of aluminium in serum by electrothermal atomic absorption spectrophotometry

Razvijena je jednostavna i osetljiva metoda za određivanje aluminijuma u serumu elektrotermalnom atomskom apsorpcionom spektrofotometrijorn. U literaturi postoje kontradiktorni podaci o analitičkim uslovima zbog čega je u ovom radu izvršeno njihovo ispitivanje. Metoda se sastoji u jednostavnom razblaživanju uzorka sa 0,05% Triton X-100 i atomizaciji uzorka na zidu pirolitički presvučene kivete. Osetljivost metode je oko 1 µg/L, a preciznost < 11 %. Za kalibraciju se preporučuje metoda dodatka standarda, ali je također moguće koristiti baždarn krivu konstruisanu dodacima aluminijuma u serum sa niskom koncentracijom ovog elementa.A simple and sensitive method for the determination of aluminium in human serum by electrothermal atomic absorption spectrophotometry has been developed. In view of contradictory data in literature all relevant analytical conditions have been tested. The method involves simple dilution with a 0.05% solution of Tri ton X-100 and atomization on the wall of a pyrolytically coated graphite tube. The sensitivity of the method is about 1 µg/L and precision < 11 %. The method of standard addition is recommended for calibration, but the use of a calibration curve based on scrum with a low content of aluminium is also possible