A correlation of the LDH isoenzymatic activity of the radicular cyst content with cytologic and microbiologic analyses

Cilj ovoga rada bio je metodom elektroforeze na poliakrilamidnom gelu izdiferencirati pojedine izoenzime laktatdehidrogenaze iz uzoraka sadržaja radikularnih cista te pri tome ispitati postojanje korelacije između izoenzimatskog spektra sadržaja sa citološkom i mikrobiološkom analizom istog. Cjelokupna istraživanja su urađena na 9 uzoraka. Biokemijski dio uređen je na Institutu za biohemiju po metodi Bloemendaala, citološki na Institutu za patologiju, a mikrobiološki u specijalističkom laboratoriju Instituta za mikrobiologiju »Dr Robert Fried« u Sarajevu. Preliminarni biokemijski rezultati ukazuju da je frakcija LDH3 prosječno više zastupljena u odnosu na LDH1, LDH-2 i LDH4 i 5. Distribucija relativnih vrijednosti izoenzima LDH u sadržaju ispitivanih radikularnih cista najsličnija je onoj u skeletnim mišićima. Citološkom analizom materijala iz radikularnih cista nađene su deskvamirane epitelne ćelije nejasnih kontura, eritrociti, bez upalnih celularnih elemenata. Mikrobiološkom analizom od 9 uzoraka u 8 je nađena isključivo aerobna mikroflora s predominacijom streptococcus alfa haemoliticus, dok je jedan uzorak ostao sterilan. Anaerobne bakterije nisu izolirane ni u jednom uzorku. Povećanjem udjela putridnih masa u sadržaju radikularne ciste te uz izolaciju fakultativnog anaeroba, pada aktivnost brzih frakcija LDH, a raste aktivnost sporih, osobito LDH;i. To ukazuje na to da su metabolički uvjeti u radikularnoj cisti pomaknuti k anaerobiji.The aim of this study was to differentiate individual lactic dehydrogenase isoenzymes from the samples of the content of radicular cysts on a polyacrylamide gel using the method of electrophoresis, and to assess thereby the existence of correlation of the content isoenzymatic spectrum with its cytologic and microbiologic analyses. The study was carried out in 9 samples. Biochemical analyses were done at the Institute of Biochemistry using the method of Bloemendaal, cytologic analyses were conducted at the Institute of Pathology, whereas microbiologic examinations were performed at the spcialized laboratory Dr Robert Fried Institute of Microbiology in Sarajevo. The preliminary biochemical results have shown the LDH3 fraction to prevail on an average in relation to LDHlf LDH2 and LDH4 and 5. The distribution of the relative LDH isoenzyme values in the content of radicular cysts is most alike that found in skeletal muscles. Desquamatory epithelial cells of blurred contours and erythrocytes but no inflamatory cellular elements were detected by the cytologic analysis of the radicular cyst material. In 8 out of 9 samples, exclusively aerobic microflora with the predominance of streptococcus alpha haemolyticus, was found by the microbiologic analysis, whereas one sample reamined sterile. Anaerobic bacteria were not isolated in any of the samples studied. Along with an increase in the proportion of putrid masses in the content of radicular cysts and isolation of a facultative anaerobe, the activity of rapid LDH fractions decreases, whereas that of the slow ones, particularly of LDH3, increases, pointing to the fact that metabolic conditions in the radicular cyst have shifted towards anaerobism