Electron microanalysis of the loss of calcium and phosphorus in initial manifestations of human enamel caries

Po četiri uzorka intaktne humane cakline 18 zuba uložena su u parcijalne proteze dobrovoljaca. Uzorci su bili izloženi djelovanju oralnih karijesogenih faktora 7, 14 21 i 28 dana. Elektronskom mikroanalizom ispitivan je demineralizacijski gubitak koncentracije kalcija i fosfora u eksperimentalnim uzorcima u odnosu na originalnu caklinu kontrolnih uzoraka. Rezultati su pokazali značajan i ravnomjeran pad koncentracije i Ca i P u svim uzorcima, ali i direktno proporcionalan vremenskom intervalu koji su uzorci proveli u ustima. Kod četiri uzorka 28-dnevne oralne eksponiranosti zabilježen je porast koncentracije i Ca i P u najpovršinskijem sloju cakline, što je protumačeno kao aktiviranje procesa remineralizacije.Sets containing of four specimens of intact human enamel of 18 teeth were inserted into volunteers’ partial prostheses. The specimens were exposed to the action of oral cariogenic factors during 7, 14, 21 or 28 days. Electron microanalysis was employed to assess the demineralizing loss of calcium and phosphorus concentrations in experimental specimens, in relation to the original enamel of control specimens. Results showed a significant and steady decrease in Ca and P concentrations in all specimens, which was directly proportional to the duration of specimen exposure to oral cavity conditions. In four specimens with a 28-day oral exposure, an increase in Ca and P concentrations was observed in the most superificial enamel layer, which was explained as activation of the process of remineraliazation