A Cytotoxic and Genotoxic Study of Magnetic Sm C05 Alloys

Istraživana je citotoksičnost i genotoksičnost samarij kobalt slitine na eukariotskom indikator organizmu Saccharomyces cerevisiae D7 u in vitro sistemu i u in vivo sistemu mikronukleus- testom na koštanoj srži štakora. Slitine su inkubirane u 0,01 M fosfatnom puferu na 37°C kroz 48 sati. Stanice iz eksponencijalne i rane stacionarne faze rasta separatno su inkubirane u opisanom puferu na 37°C kroz 2 sata u aerobnim uvjetima. Nakon tretmana stanice su isprane i resuspendirane u puferu, zasijavane na čvrstu hranjivu podlogu za ocjenu preživljavanja i na selektivnu podlogu za ocjenu frekvencije mutacije. Za analizu mikronukleus-testom dvokratno su intraperitonealno injicirani štakori s 2 ml pufera prethodno inkubiranog sa slitinom. Nakon 24 sata životinje su žrtvovane. Na temelju dobivenih rezultata može se zaključiti da ispitivane slitine koje se primjenjuju kao sredstvo retencije u mobilnoj protetici nemaju citotoksične efekte dok su genotoksični efekti u granicama greške eksperimenta.Cytotoxicity and genotoxicity of the samarium cobalt alloys were studied on an eukaryotic indicator organism of Saccharomyces cerevisiae D7 in an in vitro system and by a micronucleus test on rat bone marrow in an in vivo system. Alloys were incubated in a 0.01 M phosphate buffer at 37°C for 48 hours. Cells from exponential and early stationary phases of growth were separately incubated in the buffer at 37°C for 2 hours in aerobic conditions. After treatment, cells were washed and resuspended in the buffer, seeded on solid nutritive medium for survival assessment, and on selective medium for evaluation of the frequency of mutation. For micronucleus test analysis, rats were twice intraperitoneally injected with 2 ml of the buffer previously incubated with the alloy. Animals were sacrificed after 24 hours. Results obtained suggested the alloys, used as a tool of retention in mobile prosthetics, to elicit no cytotoxic effects, whereas genotoxic effects were within the allowed limits of experimental error