Istodobna pojava zarazne bolesti Fabricijeve burze, aflatoksikoze i sekundarnih infekcija u tovnih pilića.

A deadly outbreak of aflatoxicosis, as well as infectious bursal disease (IBD), in two adjoining organized poultry farms, further complicated by secondary microbial invasion, was investigated. Morbidity and mortality was 80-90% among broiler chicks in the 4 to 6 week age group. Samples of vital organs comprising liver, spleen, kidney, bursa of Fabricius, lungs, heart blood, bile and intestines were processed microbiologically. Morbid materials yielded Escherichia coli, Streptococcus faecalis and Proteus spp. Aspergillus flavus was isolated from feed samples collected randomly from feed stocks. Representative specimens of liver, spleen, bursa and kidney collected at 10% formal saline were subjected to histopathological investigations. Agar gel precipitation test conducted on the bursal tissue homogenates of 22 birds discerned strong precipitation lines with known positive IBD hyperimmune serum. The aflatoxin level in feed offered to the affected birds varied from 100 ppb to 500 ppb. Conspicuous histomorphological alterations corresponding to aflatoxicosis, infectious bursal disease, as well as other secondary bacterial infections, were evident in these visceral organs.Opisana je istodobna pojava aflatoksikoze popraćene uginućem, zarazne bolesti Fabricijeve burze te sekundarnih infekcija na dvije farme tovnih pilića. Pobol i pomor u pilića u dobi od 4 do 6 tjedana iznosio je 80-90%. Mikrobiološka pretraga bila je provedena na uzorcima jetre, slezene, bubrega, Fabricijeve burze, pluća, krvi iz srca, žuči i crijeva. Izdvojene su bakterije Escherichia coli, Streptococcus faecalis, Proteus spp. Iz uzorka hrane izdvojena je gljivica Aspergillus flavus. Uzorci tkiva jetre, slezene, burze i bubrega pretraženi su patohistološki. Gel difuzijskim precipitacijskim testom dokazane su jake linije precipitacije između homogenata tkiva Fabricijeve burze (22 životinje) i hiperimunog seruma za virus zarazne bolesti Fabricijeve burze. Patohistološki nalaz odgovarao je za aflatoksikozu, zaraznu bolest Fabricijeve burze i sekundarne infekcije. Razina aflatoksina u hrani kretala se između 100 i 500 ppb