Molekularna dijagnostika i serološka istraživanja pestivirusa u ovaca.

In this research, the virological and serological presence of pestiviruses, such as border disease virus (BDV) was investigated in a sheep in the Central and Eastern Black Sea Regions of Turkey. The study material consisted of 40 organ materials collected from 13 aborted lambs, which were suspected to have pestiviruses, BDV and Bovine Viral Diarrhoea Virus (BVDV). Viral nucleic acids were investigated by using the reverse transcription polymerase chain reaction (RT-PCR). Nine of 13 aborted lambs (69.2%) and 24 of 40 organ samples (60%) obtained from those sheep were BDV RNA positive, whilst all the animals and organ samples were BVDV-RNA negative. Serum samples collected from 401 randomly selected sheep were investigated for pestivirus antibodies using competitive ELISA (cELISA) and the serum microneutralisation test (SNT) using BVDV-NADL strain. Seropositivity was found in between 7.22 and 74.38% with cELISA and 4.81 and 67.76% with SNT. Seropositivity rates in Amasya and Tokat provinces were higher than in Samsun and Giresun. The obtained data indicated that pestivirus infection in sheep is widespread in the Central and Eastern Black Sea Region.U radu su prikazana virološka i serološka istraživanja pestivirusa odnosno borderske bolesti u ovaca na središnjem i istočnom području Crnoga mora u Turskoj. Ukupno je bilo pretraženo 40 uzoraka tkiva različitih organa uzetih od 13 pobačenih plodova pod sumnjom da je uzročnik bio virus borderske bolesti ili virus virusnog proljeva goveda. Uzorci su bili pretraženi na prisutnost virusne nukleinske kiseline lančanom reakcijom polimerazom uz prethodnu reverznu transkripciju (RT-PCR). Devet od 13 pobačenih plodova (69,2%) i 24 od 40 uzoraka tkiva organa (60%) sadržavalo je RNA virusa borderske bolesti. Svi pretraženi uzorci bili su negativni na virus virusnoga proljeva. Uzorci seruma nasumce prikupljeni od 401 ovce bili su pretraženi na prisutnost specifičnih protutijela uporabom kompetitivnoga imunoenzimnoga testa (cELISA) i serum neutralizacijskoga testa (SNT) sa sojem NADL virusa virusnoga proljeva goveda. Ustanovljeno je da sepostotak serološki pozitivnih cELISA-om kretao od 7,22 do 74,38%, a SNT-om od 4,81 do 67,76%. Postotak serološki pozitivnih životinja u provincijama Amasya i Tokat bio je veći nego u provincijama Samsun i Giresun. Rezultati upućuju na zaključak da su pestivirusne zaraze rasprostranjene u ovaca na središnjem i istočnom području Crnoga mora