Volumetrijski nalazi lebdećih spora gljivica u unutrašnjosti i izvan peradarnika i staja u iranskoj provinciji Mazandaran

The aim of this study was to assess the volume of airborne fungi in the indoor and outdoor environment of poultry and cattle houses in the Mazandaran Province in Iran. Indoor and outdoor air of twenty cattle houses and twenty-fi ve poultry houses were sampled using a single-stage impactor, which draws air at 20 L min-1 and impacts sampled material onto Petri plates containing malt extract agar. The plates were incubated at 30 °C for seven days, after which the resulting colonies were counted. The fungi were identified and counted microscopically and macroscopically. A total of 4,662 fungal colonies were isolated from 90 plates collected from indoor and outdoor air of cattle and poultry houses. Cladosporium (55.3 %), yeast (10.0 %), and Aspergillus (9.4 %) were the most common fi ndings. The concentration of airborne fungi in cattle and poultry houses ranged from 10 CFU m-3 to 1700 CFU m-3 in indoor and 10 CFU m-3 to 2170 CFU m-3 in outdoor environments. Cladosporium had the highest mean indoor (424.5 CFU m-3) and outdoor (449.7 CFU m-3) air concentration in the cattle houses. In the poultry houses, the highest mean concentrations were measured for Cladosporium (551.0 CFU m-3) outdoors and yeast (440.7 CFU m-3) indoors. These levels might present an occupational risk, but threshold levels for these environments have yet to be established worldwide.Cilj je ovog ispitivanja bio utvrditi razine gljivica u zraku u unutrašnjosti i izvan peradarnika i staja u iranskoj provinciji Mazandaran. Uzeti su uzorci zraka iz unutrašnjosti i izvan prostora dvadeset staja i dvadeset i pet peradarnika s pomoću jednostupanjskog impaktora s protokom zraka od 20 L min-1. Uzorkovan je zrak impaktiran na Petrijeve pločice s hranjivom podlogom od ekstrakta slada. Pločice su inkubirane sedam dana na 30 °C, a zatim su izolirane i prebrojene dobivene kolonije mikroskopski i makroskopski. Ukupno su izolirane 4.662 kolonije s 90 pločica. Najčešće su bile gljivice Cladosporium (55,3 %), kvasac (10,0 %) i Aspergillus (9,4 %). Koncentracije gljivica nošenih zrakom kretale su se od 10 CFU m-3 do 1.700 CFU m-3 u unutrašnjosti staja i peradarnika te od 10 CFU m-3 do 2.170 CFU m-3 izvan njih. Najviša srednja koncentracija u unutrašnjosti (424,5 CFU m-3) i izvan staja (449,7 CFU m-3) izmjerena je za Cladosporium. U peradarnicima najviše su srednje koncentracije u unutrašnjosti i izvan njih izmjerene za Cladosporium (551,0 CFU m-3) i kvasac (440,7 CFU m-3). Te koncentracije mogu biti povezane s rizikom od profesionalnih respiracijskih bolesti, ali još uvijek nisu utvrđene gornje dopuštene razine za ovu vrstu okoliša bilo gdje u svijetu