Učinak oralnog dodatka manganova klorida na težinu invazije vrstama Trypanosoma brucei i Trypanosoma congolense u štakora

Eighty healthy adult albino rats of both sexes were used in two experiments to study the effects of manganese chloride supplementation on the severity of Trypanosoma brucei and Trypanosoma congolense infections. In each experiment, forty rats were divided into four groups of 10 each: A. Infected unsupplemented; B. Infected supplemented; C. Uninfected unsupplemented control; D. Uninfected supplemented control. Aqueous solution (5%) of MnCl2 was administered daily using stomach tube to each rat at 50 mg/kg body weight in groups B and D from 10 days before infection to the end of the experiment. Each rat in groups A and B was infected by intraperitoneal injection of 1x106 trypanosomes (T. brucei or T. congolense) in diluted donor blood. The prepatent periods were shorter (P<0.05) in T. brucei than T. congolense infections, and shorter (P<0.05) in infected unsupplemented than in infected supplemented rats. The infected unsupplemented groups had higher (P < 0.05) parasitaemia and more severe anaemia than the infected supplemented groups. Therefore, oral manganese chloride supplementation in rats appeared to reduce the severity of trypanosome infections by delaying the onset of parasitaemia, reducing the levels of parasitaemia and accompanying anaemia.Osamdeset zdravih odraslih albino štakora oba spola rabljeni su za istraživanje učinka dodatka manganova klorida na težinu invazije vrstama Trypanosoma brucei i Trypanosoma congolense. Istraživanje je provedeno u dva pokusa. Za svaki pokus uzeto je 40 štakora podijeljenih u četiri skupine po deset. Štakori skupine A bili su invadirani, ali nisu dobivali manganov klorid. Štakori skupine B bili su invadirani i dobivali su manganov klorid. Štakori skupine C nisu bili invadirani niti su dobivali manganov klorid, dok su oni skupine D ostali neinvadirani s dodatkom manganova klorida i služili su kao kontrola.Vodena otopina (5%) MnCl2 davana je peroralano u dnevnoj dozi od 50 mg/kg tjelesne mase štakorima skupine B i D od 10. dana prije invadiranja do kraja pokusa. Svaki štakor skupina A i B bio je invadiran razrijeđenom krvi donora intraperitonealno s 106 tripanosoma (T. brucei ili T. congolense). Prepatentni period bio je kraći (P<0,05) u štakora invadiranih vrstom T. brucei nego u štakora invadiranih vrstom T. congolense. Također je bio kraći (P<0,05) u invadiranih koji nisu dobivali manganov klorid nego u onih invadiranih koji su ga dobivali. Invadirane skupine koje nisu dobivale manganov klorid imale su duže razdoblje parazitemije (P