SEPARIRANJE I ODREĐIVANJE ENANTIOMERA AMINOKISELINA KOJE SADRŽE SUMPOR POMOĆU TEKUĆE KROMATOGRAFIJE VISOKE PERFORMANCE

Performic acid oxidation of cysteine and methionine resulting in the formation of cysteic acid and methionine sulphon has been applied in order to avoid the loss of these two sulphur containing amino acids during the acidic hydrolysis of proteins that is necessary prior to amino acid analysis. The aim of the research was assigned by the increasing demand for the determination of the amount of amino acid enantiomers: the applicability of performic acid oxidation was evaluated from this point of view. Racemization of L-cysteine and L-methionine was found not significant during oxidation with performic acid, therefore this process can be applied before hydrolysis during quantification of cysteine and methionine enantiomers. Additionally, the quantification of cysteic acid and methionine sulphon enantiomers was accomplished in the form of their diastereoisomer derivatives via the development of a reversed phase of high performance liquid chromatography method.Proces oksidacije performičnom kiselinom cisteina i metionina, što rezultira stvaranjem cisteične kiseline i sulfona metionina primijenjen je kako bi se izbjegao gubitak te dvije aminokiseline koje sadrže sumpor, za vrijeme acidične hidrolize bjelančevina, potrebne prije analize aminokiselina. Cilj istraživanja potakli su sve veći zahtjevi za određivanjem količina enantiomera aminokiselina: primjenjivost oksidacije performične kiseline ocijenjena je s tog gledišta. Racemizacija L-cisteina i L-metionina nije se pokazala značajnom za vrijeme oksidacije s performičnom kiselinom pa se, stoga, taj proces može primijeniti prije hidrolize tijekom kvantifikacije cisteinskih i metioninskih enantiomera. Osim toga, kvantifikacija cisteične kiseline i enantiomera metionskog sulfona izvedena je u obliku derivata njihovih dijastereosomera putem razvoja obrnute faze metode tekuće kromatografije visoke performance