Određivanje alergena grinja u kućnoj prašini enzim-imunokemijskom metodom

The aim of this study was to determine the level of two major mite allergens Dermatophagoides pteronyssinus (Der p 1) and Dermatophagoides farinae (Der f 1) in 30 urban homes in Zagreb, Croatia, using the enzyme immunoassay with two monoclonal antibodies which has been established as the reference method for indoor allergen analysis. Dust samples were taken by vacuuming a carpeted area and collected on cellulose filters. The ranges of Der p 1 and Der f 1 were (0.1-12.5) µg g-1 (median 0.32 µg g-1) and (0.1-31.2) µg g-1 (median 0.35 µg g-1), respectively. Der p 1 and Der f 1 (>2 µg g-1) associated with increased risk of sensitization to mite allergens were found in approximately 16 % homes for each allergen. The sum of allergen (Der p 1 + Der f 1) exceeded the lower threshold in 27 % of homes. Analytical evaluation of the ELISA assay showed satisfactory results for precision (intra-assay CV 2 µg g-1) u kućnoj prašini povezana je s povećanim rizikom od senzibilizacije alergenima grinja. U ovom radu je u oko 16 % stanova ustanovljena ta razina, i to za oba alergena. Ako se zbroje maseni udjeli alergena (Der p 1 + Der f 1), tada je razina alergena u kućnoj prašini >2 µg g-1 nađena u oko 27 % stanova. Analitičkom procjenom metode ELISA dobiveni su zadovoljavajući rezultati za preciznost u seriji (KV < 6,9 %), preciznost iz dana u dan (KV < 13,3 %), točnost (91 % do 93 %) te osjetljivost (2 ng mL-1). Metoda ELISA čini nužnu bazu za naša buduća ispitivanja alergena unutarnjih prostora