Butirilkolinesteraza štiti štakore od inhalacijske izloženosti subletalnim dozama sarina

Protection experiments were conducted using different doses of equine serum butyrylcholinesterase (Eq BuChE) as pretreatment in rats. Cholinesterase activities were determined in blood [whole blood, red blood cells (RBC) acetylcholinesterase (AChE), and plasma BuChE] before and after sarin inhalation exposure in untreated rats and those pretreated with Eq BuChE. Brain AChE activity was also determined in the frontal cortex, basal ganglia and pontomedullar areas following exposure. Dose-dependent increases in plasma BuChE activity and no changes in the RBC and brain AChE activities were demonstrated following i.p. injection of different amounts of Eq BuChE. Decreases in plasma BuChE activity and RBC and brain AChE activities were observed in control rats following sarin inhalation exposure. In rats pretreated with Eq BuChE this inhibition was lower than in control animals. These results demonstrate protective effects of Eq BuChE pretreatment in rats intoxicated with sublethal concentrations of sarin by inhalation.U ovome su radu na štakorima istražene mogućnosti profilakse butirilikolinesterazom iz konjskog seruma (Eq BuChE) u različitim dozama. Aktivnost kolinesteraza izmjerena je u krvi (punoj krvi, eritrocitima i plazmi) prije i nakon inhalacije sarina u štakora koji su prethodno primili Eq BuChE. Također je nakon izlaganja sarinu mjerena aktivnost acetilkolinesteraze u tkivu uzetom iz čeonog režnja mozga štakora, bazalnih ganglija i pontomedularnog područja. Nakon primjene različitih doza Eq BuChE utvrđen je porast aktivnosti butirilkolinesteraze u plazmi koji je odgovarao dozi, ali nije bilo promjena u aktivnosti acetilkolinesteraze u eritrocitima i moždanom tkivu. U kontrolnih je štakora zamijećen pad aktivnosti butirilkolinesteraze u plazmi te acetilkolinesteraze u eritrocitima i moždanom tkivu nakon njihova izlaganja sarinu inhalacijskim putem. U štakora koji su primili profilaksu Eq BuChE ova je inhibicija bila slabija. Rezultati istraživanja potvrđuju zaštitna svojstva konjske butirilkolinesteraze kao profilakse pri otrovanju štakora subletalnim dozama sarina