The Antimicrobial Effect of Calasept, Superlux Calcium Hydroxide Liner and Gutta-percha with Calcium Hydroxide

Mikroorganizmi i učinci njihova metabolizma glavni su uzročnici patoloških promjena u zubnoj pulpi i u periradikularnom tkivu. Svrha našega istraživanja bila je in vitro odrediti bakterijsku učinkovitost Calasepta (Speico, Darmstadt, Njemačka), Superlux calcium hydroxidelinera (Dental Material Gesellschaft, Hamburg, Njemačka) i Calcium hydroxide conesa (ROEKO Langenau, Njemačka) u izravnom doticaju s mikroorganizmima. Uporabljeni su mikroorganizmi Enterococcus faecalis (ATCC 29212) i Candida albicans (NCTC 3123). Pripravljena je prekonoćna miješana kultura mikroorganizama (108 CFU/ml). U tu suspenziju na 5 je minuta uronjeno ukupno 36 absorbirajućih sterilnih papirnih konusa N 50 (ROEKO Langenau, Njemačka) i 18 gutaperka konusa presvučenih kalcijevim hidroksidom (ROEKO Langenau, Germany). Konusi su nakon toga smjeπteni u petrijevke (7). Papirni štapići tada su pažljivo prekriveni s Calaseptom ili Superlux calcium hydroxide linerom. U razmacima od 0, 6, 12, 24, 48 i 72 sata tri “pointa” od svake ispitne skupine su uklonjena iz doticaja s pastom kalcijeva hidroksida i uronjeni u soja triptoza bujon. Inkubirani su 72 h na 37°C, aerobno. Antimikrobni učinak Calasepta i Superlux Calcium hydroxid linera pokazao se nakon 6 h za Enterococcus faecalis i nakon 12 h za Candidu albicans. Antimikrobmo djelovanje gutaperka štapića prekrivenih kalcijevim hidroksidom nije primijećeno nit za jedan ispitani mikroorganizam u 72 h.Microorganisms are the main cause of disease in root canals and periapically. Facultative bacteria and fungi have been identified in persistent endodontic infection. The objective of this study was to determine in vitro the efficacy of: Calasept (Speiko, Darmstadt, Germany), Superlux calcium hydroxide liner (Dental Material Gesellschaft, Hamburg, Germany) and gutta-percha with calcium hydroxide (Roeko, Langenau, Germany) in direct contact with microorganisms. The microorganisms used were: Enterococcus faecalis (ATCC 29212) and Candida albicans (NCTC 3123). An overnight mixed broth culture of microorganisms (108 CFU/ml) was prepared. Paper points and gutta-percha points were immersed in suspension for 5 min. The paper points were then covered with Calasept and Superlux calcium hydroxide liner. At intervals of 0; 6; 12; 24; 48 and 72 h at 37°C, aerobically. The antimicrobial effect of Calasept and Superlux calcium hydroxide liner apparently occured after 6h on Enterococcus faecalis and 12 h on Candida albicans. With gutta-percha, this effect did not occur for either Enterococcus faecalis or Candida albicans after 72 h