When muscle is allowed to shorten during an active contraction, the maximum force that redevelops after shortening is smaller than the isometric force at the same muscle length without prior shortening. This phenomenon is referred to as force depression (FD) following shortening contractions. The purpose of this study was to investigate the effects of muscle fatigue on shortening-induced FD in electrically activated human quadriceps muscle. Healthy untrained men (n=8) performed isometric reference contractions (ISOM) and isometric–concentric–isometric (ICI) contractions, using maximal electrical stimulation. FD was assessed by comparing the steady-state isometric forces produced following active muscle shortening with the purely isometric r...