Qualitative and quantitative determination of selected isothiocyanates and nitrilies using GC-MS/MS : bachelor thesis

Glukozinolati su fitokemikalije iz kojih hidrolizom nastaju lako hlapljivi spojevi izotiocijanati i nitrili koji zbog svoje iskazane biološke aktivnosti predstavljaju temu od interesa. Plinska kromatografija je često korištena analitička tehnika kojom se odjeljuju komponente iz uzorka. Kombinacijom plinske kromatografije s tandemskom spektrometrijom masa postoji mogućnost identifikacije i određivanja lako hlapljivih spojeva. Cilj ovog rada bio je izraditi metodu za kvalitativnu i kvantitativnu analizu fenilacetonitrila, 3-fenilpropanonitrila, 2-feniletil-izotiocijanata i izopropil-izotiocijanata. Dobiveni rezultati pokazuju da su određivanje koncentracije i identifikacija odabrane komponente iz uzorka uspješne iz čega se moţe zaključiti da je metoda valjana.Glucosinolates are phytochemicals from which the easily volatile compounds isothiocyanates and nitriles are formed by hydrolysis which represent the topic of interest due to their biological activity. Gas chromatography is a widely used analytical technique that separates components from a sample. By combining gas chromatography with mass spectrometry, we have the ability to identify and quantify volatile compounds. The aim of this work was to develop a method for qualitative and quantitative analysis of phenylacetonitrile, 3-phenylpropanonitrile, 2-phenylethyl isothiocyanate and isopropyl isothiocyanate. The obtained results show that it is necessary to determine the concentrations and identify the selected components from the sample successfully from which it can be concluded that the method is valid