Development of Antibiofilm Nanocomposites: Ag/Cu Bimetallic Nanoparticles Synthesized on the Surface of Graphene Oxide Nanosheets

There are numerous issues associated with bacteria, particularly biofilms, which exhibit a strong resistance to antibiotics. This is currently considered an urgent global issue owing to the lack of effective treatments. Graphene oxide (GO) nanosheets are two-dimensional carbon materials that are available as a substrate for metal nanoparticles and have a lower release rate of metal ions than free metal nanoparticles by regulating the oxidation of metal nanoparticles, which is known to reduce the cytotoxicity caused by the free metal nanoparticles. Over centuries, metal particles, including Ag and Cu, have been considered as antibacterial agents. In this study, Ag and Cu bimetallic nanoparticles on a GO surface (Ag/Cu/GO) were synthesized using a chemical reduction method, and their antimicrobial effects against several bacterial species were demonstrated. Ag/Cu/GO nanocomposites were characterized by transmission electron microscopy and energy-dispersive X-ray spectroscopy. The in vitro cytotoxicity of an Ag/Cu/GO nanocomposite was evaluated in human dermal fibroblasts, and its antibacterial activity against Methylobacterium spp., Sphingomonas spp., and Pseudomonas aeruginosa (P. aeruginosa) was also tested. The synthesized Ag/Cu/GO nanocomposite was able to eradicate all three bacterial species at a concentration that was harmless to human cells. In addition, Ag/Cu/GO successfully removed a biofilm originated from the culturing of P. aeruginosa in a microchannel with a dynamic flow. In a small-animal model, a biofilm-infected skin wound was healed quickly and efficiently by the topical application of Ag/Cu/GO. The Ag/Cu/GO nanocomposites reported in this study could be used to effectively remove antibiotic-resistant bacteria and treat diseases in the skin or wound due to bacterial infections and biofilm formation.restrictio