Comparison of the composition of free volatile compounds of the species Veronica chamaedrys L. isolated by microwave extraction and hydrodistillation

Vrsta Veronica chamaedrys (dvorednodlakava čestoslavica) pripada rodu Veronica (porodica Plantaginaceae). Raste na livadama i pašnjacima. Rod Veronica se dugo vremena koristi u narodnoj medicini zbog protuupalnih, antimikrobnih i antikancerogenih svojstava. Slobodne hlapljive spojeve vrsta ovog roda nalazimo u žljezdanim trihomima i izolirani su dvjema tehnikama: vodenom destilacijom (HD) u aparaturi po Clevenger-u i metodom mikrovalne ekstrakcije Ethos X aparaturom (ME). Identifikacija spojeva lipofilne i vodene faze provedena je GC/MS analizom. Najzastupljeniji spoj u obje faze i korištene dvije izolacijske tehnike je kariofilen oksid (eterična ulja: HD-18,63% ; ME-24,36%, hidrolati: HD-20,65% ; ME-20,26%) koji pripada oksigeniranim seskviterpenima. Monoterpenske ugljikovodike identificirane u sastavu hidrolata V. chamaedrys (HD-8,24%, ME-4,56%) ne nalazimo u sastavu eteričnih ulja ove vrste.Species Veronica chamaedrys (Germander speedwell) belongs to genus Veronica (Plantaginaceae family). It grows on meadows and pastures. Genus Veronica has long been used in medicine because of its anti-inflammatory, antimicrobical and anticancerogenic properties. Free volatile compounds of this genus are found in glandular trichomes. Essential oil of Veronica chamaedrys was isolated with two techniques: hydrodistillation in Clevenger apparatus and microwave extraction with Ethos X apparatus. Identification of compounds from lipophilic and water phase was performed by GC/MS analysis. Most common compound in both phases extracted with both techniques was caryophillene oxide (essential oils : HD-18,63% ; ME-24,36%, hydrolates : HD-20,65% ; ME-20,26%) that belongs to oxygenated sesquiterpenes. Monoterpene hydrocarbons found in hydrolates (HD-8,24%; ME-4,56%) are not found in essential oils