Xyloglucan and Concanavalin A based dressings in the topical treatment of mice wound healing process

For medical biomaterials, xyloglucan dispersions can form films or gels to be applied as a wound dressing. For this purpose, the structural characterization of xyloglucan dressing (XG) and xyloglucan dressing containing 0.5 mg/mL of concanavalin A (XGL) was performed. The lectin release capacity and stability, cytotoxicity, and pro-wound healing effects were also investigated. XG and XGL films were prepared by mixing 0.5 % (w/v) xyloglucan with 0.3 % (v/v) glycerol. The ConA incorporated in the xyloglucan dressing maintained its biological activity for fourteen days in a controlled-release manner. The films were non-toxic, homogeneous, flexible, and accelerated the wound contraction compared with the control group, promoting less infiltration of inflammatory cells, angiogenesis, remodeling, and early epithelization. The films also alleviate the inflammation phase by reducing the production of pro-inflammatory cytokines (IFN-, TNF-, IL-1, IL-6, and IL-12), especially the XGL film, which promoted the up- and down-regulation of important proteins associated with the wound repair. All these findings suggest that XG and XGL films may represent a good therapeutic approach for wound healing applications.The authors are grateful for the financial support for research grants from the Conselho Nacional de Desenvolvimento Científico e Tecnol ´ogico (CNPq), Coordenaç˜ao de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Fundaç˜ao de Amparo `a Ciˆencia e Tecnologia do Estado de Pernambuco (FACEPE). We are grateful to the Centro de Tecnologias Estrat´egicas do Nordeste (CETENE) and to the Laborat´orio de Imunopatologia Keizo Asami-LIKA at the Universidade Federal de Pernambuco (UFPE) for access to its installation and technical assistance.