Study of Microbial Preparation Viability on Various Natural Materials

Predmet istraživanja ovog rada je mogućnost primjene supstrata koji se nalaze u prirodi, dakle koji nisu sintetskog podrijetla, primjerice alginita, vermikulita, zeolita i dijatomejske zemlje u poboljšanju uzgoja povrtnih kultura. Navedeni supstrati bi poslužili kao nosači biomase mikroorganizama koji zbog svojih enzimatskih svojstava imaju sposobnosti aktivnih ekoloških pesticida i insekticida, te bioloških gnojiva. Mikroorganizmi koji se koriste u ovom radu su plijesni iz rodova Trichoderma i Beauveria, te bakterijske vrste Bacillus thuringiensis i Azotobacter chroococcum. Cilj rada je ispitati preživljavanje, odnosno broj živih stanica bakterija i spora plijesni, na navedenim supstratima tijekom zadanog vremena u uvjetima primjene. Kao najbolji supstrat pokazao se alginit, a najbolju održivost na njemu imala je bakterija Azotobacter chroococcum.The subject of this paper is the possibility of using substrates that are found in nature, ie not of synthetic origin, such as alginite, vermiculite, zeolite and diatomaceous earth in improving the cultivation of vegetable crops. These substrates would serve as carriers of biomass of microorganisms which, due to their enzymatic properties, have the ability of active ecological pesticides and insecticides, as well as biological fertilizers. The microorganisms used in this work are molds from the genera Trichoderma and Beauveria, and the bacterial species Bacillus thuringiensis and Azotobacter chroococcum. The aim of this study was to examine the survival, ie the number of living cells of bacteria and mold spores, on these substrates during a given time under application conditions. Alginite proved to be the best substrate, and it had the best viability on it the bacterium Azotobacter chroococcum