Application of Eutectic Solvents in the Extraction of Phlorotannins from Padina pavonica Algae and Determination of Antioxidant Activity of Extracts

Padina pavonica smeđa je alga, koju pronalazimo i u Jadranskom moru. Ova alga je pronašla široku primjenu u proizvodnji biodizela, biosorpciji teških metala, kao biomonitor metala u tragovima, bioindikator zagađenja, antioksidans, bioinsekticid te kao antibakterijsko sredstvo. U ovom završnom radu provedena je ekstrakcija na navedenoj algi sa 15 niskotemperaturnih eutektičkih otapala na bazi kolin-klorida. Ekstraktima je određen sadržaj florotanina, te antioksidacijska aktivnost određivanjem hvatanja slobodnog DPPH radikala. Na temelju dobivenih rezultata određeno je najpogodnije otapalo za dobivanje ekstrakta alge P. Pavonicas najvišim udjelom florotanina, te s najvišom antiradikalnom aktivnošću.Padina pavonica is a brown algae, which is also found in the Adriatic Sea. This algae has wide usage in the production of biodiesel, biosorption of heavy metals, as a biomonitor of trace metals, bioindicator of pollution, antioxidant, bioinsecticide and as an antibacterial agent. In this final work, extraction was performed on said algae with 15 low-temperature eutectic solvents based on choline chloride. The extracts determined the content of florotanin and antioxidant activity by determining the capture of free DPPH radicals. Based on the obtained results, the most suitable solvent for obtaining P. Pavonica algae extract with the highest content of florotanin and with the highest antiradical activity was determined