Determination of antioxidant activity of analgesics by HPLC-DPPH method

Bol je složen, neugodan senzorni i emocionalni doživljaj koji se javlja kao direktni odgovor na stvarno ili potencijalno oštećenje tkiva. Analgetici se koriste u svrhu ublažavanja i uklanjanja boli kod niza stanja i bolesti različite etiologije. Obzirom na njihovu široku i čestu primjenu, korisno je ispitati imaju li predstavnici ove skupine lijekova dodatna mehanizme djelovanja poput antioksidativnog učinka. Cilj ovog rada bio je odrediti antioksidativnu aktivnost devet analgetika u in vitro uvjetima mjerenjem njihove sposobnosti hvatanja DPPH slobodnog radikala. U ovom radu je za procjenu antioksidativne aktivnosti 1 mM otopine analgetika korištena HPLC-DPPH metoda, koja se temelji na mjerenju sposobnosti hvatanja slobodnog radikala u prisustvu ispitivanog lijeka. Antioksidativni aktivnost izražen je kao ekvivalent standarda TROLOX-a. Dobiveni rezultati ukazuju na izraženu antioksidativnu aktivnost paracetamola. Korištena HPLC-DPPH metoda pokazala se jednostavnom, brzom i učinkovitom za ispitivanje antioksidativne aktivnosti slabih analgetika s ciljem ispitivanja njihovog dodatnog mehanizma djelovanja.Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage. Analgesics are used for relieving or removing pain in number of conditions and illnesses with various etiology. Since they are widely and frequently used, it is useful to examine if they potentially have additional effects, especially antioxidant. The aim of this thesis was to examine antioxidant properties of nine nonsteroidal anti-inflammatory drugs (NSAIDs) by measuring their ability to scavenge DPPH radical in vitro. Method used for estimating antioxidant activity of 1 mM analgesic solutions in this research was HPLC-DPPH method, which is based on measuring the ability to scavenge free radicals in the presence of sample. Antioxidant capacity is expressed as the TROLOX equivalent. Experimental data indicate that paracetamol has antioxidant activity. The HPLC-DPPH method has been proven as simple, fast and efficient for antioxidant activity assays of NSAIDs with the purpose of examining their potential additional mechanisms of action