Anti-nociceptive Activity of Regenerated Drosera spatulata Aqueous Extract by Rat Formalin Test

Background: The use of regenerated plants provides a new era in herbal medicine production. Objective: In this study, the analgesic effect of aqueous extracts from aerial part of regenerated Drosera spatulata (Droseraceae) was examined on Sprague Dawley rats by formalin test. Methods: The D. spatulata cultured on hormone-free solid Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose and 0.7% (w/v) agar for 3 months. The aqueous extract prepared from aerial part of regenerated plant. Results: The extract (0.05 mg/kg, i.p.) significantly (p<0.05) and in a dose-dependent (0.05, 0.1, 0.5, 1 mg/kg, i.p.) manner reduced rat flinching responses. Sodium salicylate (300 mg/kg) was used as a positive control. Sodium salicylate (300 mg/kg, i.p.) and extract (0.05 mg/kg, i.p.) alleviated rats nociception in the second phases, while in the first phase, only the extract caused an anti-nociceptive effect (p