Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture

Summary: Microglial dynamics and interactions with nearby radial glia can be visualized in real time in embryonic mouse brain tissue using time-lapse imaging in slice culture. This live-cell imaging protocol can be used to study the morphology and activities of a number of cell types across a variety of brain regions and developmental time points. The advantage of this brain slice culture model is that it allows for the visualization of cellular interactions and movements in real time, especially across embryogenesis.For complete details on the use and execution of this protocol, please refer to Rosin et al. (2021)