Determination of pesticide residues in wine by solid-phase extraction on-line combined with liquid chromatography tandem mass spectrometry

Liquid chromatography tandem mass spectrometry (LC–MS/MS) has been combined with different sample preparation strategies to determine potential pesticide residues existing in wine. Herein, we propose the on-line coupling between solid-phase extraction (SPE), based on a reversed-phase polystyrene-divinyl benzene (PS-DVB) sorbent, and LC–MS/MS for the automated monitoring of a selection of 48 pesticides (37 fungicides and 11 insecticides) in this beverage. Parameters affecting the sensitivity, selectivity, accuracy and robustness of the method are evaluated as function of sample volume, clean-up conditions, and number of extraction-desorption cycles performed with the same SPE cartridgeL.P.M thanks a FPU grant to the Spanish Ministry of Science. This study was supported by Spanish Government and Xunta de Galicia through grants PGC2018-094613-B-I00 and ED431C 2021/06, both co- funded by the EU FEDER program. We acknowledge Agilent for providing access to the LC-ESI-MS/MS instrumentation employed in this studyS