Establishment of novel patient-derived sarcoma cell lines : preliminary results

Sarcomas are a very diverse group of rare, malignant tumors, consisting of more than 50 histological subtypes, arising in mesenchymal tissue. Despite trials with new compounds, survival rates have stagnated over the past 20 years. There is a great need for preclinical models in sarcoma research, however, there is only a limited number of in vitro disease models commercialy available for functional testing and target validation. A well accepted alternative to commercialy available cell lines is the use of primarry cell cultures. Unfortunantly, their behaviour is unpredictable with an uncertaintanty of succesfull establishment, espacially in sarcomas. In this poster, our experience so far with primary cell culture of sarcoma patients is shown. Six patient-derived single cell suspensions (two undifferentiated pleiomorphic-, one osteo- with chondroblastic differentiation, two synovial sarcomas and one malignant solitary fibrous tumor), processed at different time points from fresh tumor tissue, and stored in liquid nitrogen, were thawned and cultivated in a 6-well plate in both DMEM 10% FBS + P/S and RPMI 1640 10% FBS + P/S. After 2 months, only two cell lines have passed the 10th passage and were confirmed to be sarcoma cell lines with clear characteristics of the primary tumor by embedding a cell pellet from a T175 falcon in paraffine and performing an H/E- and immunohistochemic staining. Two other cell lines are still at the 3th passage and form monolayers without distinct colony formation. The last two cell lines are still growing slowly in loose colonies in a 6-well plate