Identification of iron by methods of cytochemical analysis in the spleen tissues during experimental radiation pathology

The article presents the results of studying the radio protective properties of alfalfa extract (dietary supplement "Erakond"). A decrease in the iron content in macrophages of the red pulp of the spleen was found experimentally using models of experimental radiation pathology induced by ɣ-irradiation of white rats in a single dose of 4 Gy at a dose rate of 1.7 Gy/min. The mechanisms of pathological processes in acute radiation pathology, leading to a decrease in the content of endogenous iron at the systemic, organ, cellular and subcellular levels, have been disclosed. Violation of the processes of enteral absorption of minerals as a result of pronounced destructive-inflammatory processes in the intestinal wall, damage to the hematopoiesis-inducing environment in the red bone marrow, inhibition of cytokine synthesis (IL-1, IL-3, IL-6, IL-7) and granulocytic macrophage colony-stimulating factor (GM-CSF) reduce the level of deposited iron in acute radiation pathology. In rats treated with 40 % "Erakond" aqueous extract before and after irradiation, there is a relatively high level of deposited iron in the spleen tissues in comparison with animals of the comparison group, which were on a general food ration