Seedling growth, physiological characteristics, nitrogen fixation, and root and nodule phytase and phosphatase activity of a low-phytate soybean line

Understanding the influence of the valuable “low-phytate” trait on soybean seedling growth, physiology, and biochemistry will facilitate its future exploitation. The aim was to elucidate the physiological and biochemical characteristics of low-phytate (LP) soybean at the seedling stage. To this end, seed P and mineral content and seedling dry weight, carbon (C) and nitrogen (N) accumulation, nitrogen fixation, and root and nodule phytase and phosphatase activity levels were measured at 21 d after sowing LP and normal-phytate (NP) soybean lines. Seedling dry weight, and C and N accumulation were 31%, 38% and 54% higher, respectively, in the LP line than the NP line. The total and specific nitrogen fixation levels in the LP nodules were 46% and 78% higher, respectively, than those in the NP nodules. The phytase, phosphatase, and specific phytase levels were 1.4-folds, 1.3-folds, and 1.3-folds higher, respectively, in the LP roots than the NP roots. The phosphatase and specific phosphatase levels in LP nodules were 1.5-folds and 1.3-folds higher, respectively, than those in the NP nodules. The mineral levels were substantially higher in the LP seeds and seedings than in those of the NP line. The HCl extractabilities of P, S, Fe, Cu and Mn were higher in the LP seeds than the NP seeds. These results indicate that the LP line presented with superior seedling growth and nitrogen fixation relative to the NP line. The LP line had relatively higher root phytase and root and nodule phosphatase activity levels than the NP line and could, therefore, be better suited and more readily adapt to low P conditions.This study was funded by Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research (KAKENHI), Japan: Grant Number 15K14678 and 18K05948 to Saneoka H., and the financial supported by China Scholarship Council(CSC), China to Mr. Qin Dong