Effect of silver nitrate on the in vitro germination of Euphorbia nutans Lag.

Euphorbia nutans Lag. it is a herbaceous plant found in the Mixteca Poblana, Mexico. It is considered a weed, it is not cultivated and its medicinal use to treat gastric problems and heal wounds is ignored. This situation can cause your loss of the region. In addition, it presents difficulties to in vitro establish from plant tissues and seeds. Therefore, the objective of this work was to determine the effect of silver nitrate (AgNO3) on the in vitro germination of E. nutans seeds in combination with factors such as the position of the seeds in the culture medium, lighting conditions and the use of growth regulators. The MS culture medium was used to compare three seed positions, lighting conditions: (16/8 h) and darkness, and 1.0 mg l-1 of BA, AG3 and AIA. The results showed that the germination of the seeds is partially favored depending on the growth regulator or lighting. However, in all cases, germination was higher with AgNO3, with 100% of seeds germinated from the first week of culture. The seedlings reached the height of the container in three weeks of culture with a significant difference with the treatments without AgNO3. Silver nitrate plays an essential role in the in vitro germination of E. nutans seeds and in the growth of the seedlings obtained. This is the first work on the in vitro management of E. nutans