Laboratory-Scale Bio-Treatment of Real Arsenic-Rich Acid Mine Drainage

International audienceAcid mine drainage (AMD) still represents a huge environmental problem. Technical and scientific breakthroughs are still needed to decrease environmental damages, treatment cost, and waste production associated with AMD. The feasibility of combining continuously fed anaerobic sulfate-reducing bioreactor with downstream iron oxidation step was tested, at a laboratory scale, with two types of real arsenic-rich AMD waters from the site of Carnoulès (France), that differed in acidity (pH 3.3 and 4.0), arsenic (As, 18 and 174 mg.L−1), and metal concentrations. Iron remained in solution, while up to 99% of As was precipitated as amorphous orpiment in the anaerobic sulfate-reducing bioreactor. Zinc (Zn) precipitation was also observed, up to 99%; however, the efficiency of Zn precipitation was less stable than that of As. The anaerobic bioreactor presented a stable bacterial community including a Desulfosporosinus-related sulfate reducer. When the effluents from the anaerobic process step were treated in a laboratory aerobic bioreactor, iron was oxidized efficiently. The feasibility of efficient orpiment bio-precipitation coupled with downstream iron oxidation was shown, thus opening the perspective of a low-cost combination of treatment steps for the removal of arsenic, zinc, and iron in As-rich AMDs