Antimutagenecity potential of isolates from the chloroform extract of Bidens Pilosa Vidal

Air dried leaves of Bidens Pilosa V. were ground and soaked in chloroform, after which the chloroform extract was concentrated in vacuo leaving a crude extract. The resulting extract was subjected to gravity liquid chromatography (GLC). Fractions obtained from the GLC were monitored by thin layer chromatography (TLC). The fractions were rechromatographed until the isolates were obtained. The isolates including a novel diterpene from Ms. Carmelita Zulueta were tested for possible antimutagenic activity by micronucleus test. Results of the study indicated that the isolates have antimutagenic properties