Amperometric alcohol biosensors based on conducting polymers: Polypyrrole, poly(3,4-ethylenedioxythiophene) and poly(3,4-ethylenedioxypyrrole)

Alcohol biosensors based on conducting polypyrrole (PPy), poly(3,4-ethylenedioxythiophene) (PEDOT) and poly(3,4-ethylenedioxypyrrole) (PEDOP) were constructed. Alcohol oxidase (AlcOx, from Pichia pastoris) was immobilized during electropolymerization of the monomers in sodium dodecylsulfate (SDS) and phosphate buffer electrolysis medium. Optimization of several parameters was carried out. The highest affinity was observed for the PEDOT/AlcOx sensor. Lowry protein determination method was also used to calculate the amount of immobilized enzyme in sensors. Before testing the biosensors on alcoholic beverages effects of interferents (glucose, acetic acid, citric acid, and I.-ascorbic acid) were determined. The alcohol contents of the distilled beverages (vodka, dry cin, whisky, and rain) were determined with the sensors constructed. A good match with the chromatography results was observed