The objectives of this thesis are investigation of, i) the effects of oxygen transfer conditions on recombinant protein production in Pichia pastoris strains designed with novel naturally occuring pyruvate kinase (PYK) promoter (PPYK) which is a potential promoter for recombinant protein production under low to moderate oxygen transfer conditions, and ii) influences of engineering with single- and multi- copy genes, in fed-batch fermentation processes. Production of recombinant human growth hormone (rhGH) by P. pastoris strains under PPYK carrying single- and multi- copies of hGH gene were carried out to determine the effect of gene copy number. For the characterization of the performances of the engineered P. pastoris strains, effects of t...
Cell cycle synchronization used as a common procedure for understanding various genomic and metaboli...
Growth of the biopharmaceutical market and advances in host strain engineering have fuelled the appl...
BACKGROUND: The yeast Pichia pastoris is widely used as a production platform for secreted recombina...
The aim of the M.Sc. thesis is expression of recombinant human growth hormone (rhGH) by Pichia pasto...
In this study, the effects of bioprocess operation parameters on recombinant human growth hormone (r...
The objective of this study is to investigate different feeding strategies leading to higher recombi...
Glyceraldehyde-3-phosphate dehydrogenase promoter, PGAP, is known to be a strong con...
Novel strong Pichia pastoris promoter discovery is crucial in metabolic engineering for recombinant ...
The aim of this study was to develop an effective feeding strategy to enhance recombinant human grow...
The aim of this study is to investigate effects of oxygen transfer conditions on recombinant glucose...
The objective of the current research was to develop a recombinant Pichia pastoris (r-P. pastoris) s...
Novel strong promoter discovery is crucial for the design of novel strains of the industrial yeast P...
In this study, the aim was to investigate the effects of pH on therapeutically important protein, re...
The combination of strain and bioprocess engineering strategies should be considered to obtain the h...
BACKGROUND: The influence of methanol feed rate on recombinant human growth hormone (rhGH) productio...
Cell cycle synchronization used as a common procedure for understanding various genomic and metaboli...
Growth of the biopharmaceutical market and advances in host strain engineering have fuelled the appl...
BACKGROUND: The yeast Pichia pastoris is widely used as a production platform for secreted recombina...
The aim of the M.Sc. thesis is expression of recombinant human growth hormone (rhGH) by Pichia pasto...
In this study, the effects of bioprocess operation parameters on recombinant human growth hormone (r...
The objective of this study is to investigate different feeding strategies leading to higher recombi...
Glyceraldehyde-3-phosphate dehydrogenase promoter, PGAP, is known to be a strong con...
Novel strong Pichia pastoris promoter discovery is crucial in metabolic engineering for recombinant ...
The aim of this study was to develop an effective feeding strategy to enhance recombinant human grow...
The aim of this study is to investigate effects of oxygen transfer conditions on recombinant glucose...
The objective of the current research was to develop a recombinant Pichia pastoris (r-P. pastoris) s...
Novel strong promoter discovery is crucial for the design of novel strains of the industrial yeast P...
In this study, the aim was to investigate the effects of pH on therapeutically important protein, re...
The combination of strain and bioprocess engineering strategies should be considered to obtain the h...
BACKGROUND: The influence of methanol feed rate on recombinant human growth hormone (rhGH) productio...
Cell cycle synchronization used as a common procedure for understanding various genomic and metaboli...
Growth of the biopharmaceutical market and advances in host strain engineering have fuelled the appl...
BACKGROUND: The yeast Pichia pastoris is widely used as a production platform for secreted recombina...