Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed-batch operation

BACKGROUND: The influence of methanol feed rate on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) in medium containing sorbitol was investigated at three different specific growth rates (mu), namely, 0.02 (MS-0.02), 0.03 (MS-0.03), and 0.04 (MS-0.04)