Intestinal sepsis model of small animals

Objective: The goal of this paper was to develop a model of intestinal sepsis in the investigational animal. Material and methods. Sprague-Dawley male rats of 4 weeks (N = 42) were used. Initially, a pilot study (N = 12) was performed and distributed in groups with .8 cc inoculum of Escherichia coli ATCC intraperitoneally administered at concentrations of 9, 7, 6. Subsequently, concentrations of 9 CFU are used in two groups of rats with reductions of 11 cc.  Finally, a randomized trial of 28 rats was initiated in three treatment groups after intraperitoneal infection. Biological models of blood and peritoneal fluid were observed, and histopathological study of intraperitoneal tissues was done. Results. Demise of 95% of the rats infected with E. coli UFC concentration was observed. The blood culture and peritoneal fluid culture was positive for the same strain in all of them. The formation of blisters on the liver surface and polymorphonuclear infiltration in organs were detected. Conclusion. The fatal dose of E. coli should be diluted for intraperitoneal injection