Stability indicating simultaneous validation of hydrochlorothiazide, dihydralazine, and propranolol with thestudy of forced degradation behavior through RP-HPLC in thepharmaceutical dosage form

For the instantaneous assay of Hydrochlorothiazide, Dihydralazine and Propranolol in tablet, an easy, accurate and truthful RP-HPLC method have been developed as well as validated. Isocratic RP-HPLC method was developed on BDS hypersil C18, (250mm×4.6mm internal diameter, 5µ particle size) using mobile phase as 0.05M Potassium Dihydrogenortho Phosphate (pH-6.0): Acetonitrile (40:60v/v) at a 1.0 mL/min flow rate than at 215 nm detection was carried out using tunable absorbance detector (Waters 486). Study of forced degradation study was carried out through acid degradation, base degradation, thermal degradation, oxidation of the drug. The validation of method was carried out to observe accuracy, precision, linearity, in addition to robustness. The method was found to be linear in the concentration range of 10-3