Gastroprotective Effect of DLBS2411 Bioactive Fraction from Cinnamomum burmannii Against Ethanol-Induced Gastric Damage in Rats

Raymond R Tjandrawinata,1,2 Florensia Nailufar1 1Dexa Laboratories of Biomolecular Sciences, Dexa Medica, Cikarang, West Java 17550, Indonesia; 2Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Tangerang 15345, IndonesiaCorrespondence: Raymond R TjandrawinataDexa Laboratories of Biomolecular Sciences, Dexa Medica, Industri Selatan V Block PP No. 7, Jababeka Industrial Estate II, Cikarang, West Java 17550, IndonesiaTel +62 21 89841901Fax +62 21 8984 1905Email raymond@dexa-medica.comBackground: The study was carried out to evaluate the anti-ulcerative and gastroprotective effect of DLBS2411, a bioactive fraction from Cinnamomum burmannii (Nees & T. Nees) Blume, in Wistar rats (Rattus norvegicus).Methods: The rats were divided into five treatment groups, which were the Normal control group, Negative control group (ethanol-induced) and two treatment groups: DLBS2411 at the doses of 25 mg/kg body weight (BW) and 50 mg/kg BW, and the Positive control group treated with sucralfate at the dose of 100 mg/kg BW. Gastroprotective effect was measured by the ulcerative lesion index, ulcer surface area, percentage of lesion area, and cure ratio. Hematological and histopathological analyses were also conducted to gain additional data regarding the gastroprotective effect of DLBS2411 in the rats’ stomachs.Results: DLBS2411 was found to contain not less than 15% of total phenolic compounds. Treatment with DLBS2411 at doses of 25 mg/kg BW and 50 mg/kg BW significantly reduced the percentage of ulcer area in rats. The percentage of ulcer area for the Negative control group and both doses in the DLBS2411 treatment group reached 22.64± 6.82%, 6.75± 4.41%, and 6.18± 4.63%, respectively. Ulcer surface area in the treatment groups and Positive control group also decreased. Histopathological data showed that gastric epithelial cells in the Negative control group were more severely ulcerated than in the treatment group of DLBS2411 and the Positive control group.Conclusion: This study showed that DLBS2411 at the dose of 50 mg/kg BW was more effective in protecting the stomach lining than DLBS2411 at the dose of 25 mg/kg BW, as measured by percentage of ulceration inhibition and the ulcerative lesion index.Keywords: gastroprotective, ethanol, Cinnamomum burmannii, DLBS2411, ra