Resveratrol encapsulation in high molecular weight chitosan-based nanogels for applications in ocular treatments: Impact on human ARPE-19 culture cells
- Buosi, Florencia Solana
- Alaimo, Agustina
- Di Santo, Mariana Carolina
- Elias, Fernanda
- Garcia Liñares, Guadalupe Eugenia
- Acebedo, Sofía Lorena
- Castañeda Cataña, Mayra Alejandra
- Spagnuolo, Carla Cecilia
- Lizarraga, Leonardo
- Martínez, Karina Dafne
- Perez, Oscar Edgardo
DOIAbstract
Oxidative stress and inflammation play a pivotal role in ocular diseases. Resveratrol (RSV) is a natural bioactive that has recently attracted attention due to it potent antioxidant and anti-inflammatory properties. However, RSV presents poor aqueous solubility and chemical instability. Besides, effective drug delivery to the posterior segment of the eye is challenge. Nanotechnology emerges as a possible solution to improve both limitations. Here, we developed and characterized nanogels (NG) based on high molecular weight chitosan (HCS) crosslinked with sodium tripolyphosphate. The distribution size of NG presented a major population around 140 nm with a ζ-potential value of 32 ± 2 mV. TEM and AFM images showed that NG exhibited a rounded m...