Regulatable adenovector harboring the GFP and Yamanaka genes for implementing regenerative medicine in the brain
- Lehmann, Marianne
- Canatelli Mallat, Martina
- Chiavellini, Priscila
- Morel, Gustavo Ramón
- Reggiani, Paula Cecilia
- Hereñú, Claudia Beatriz
- Goya, Rodolfo Gustavo
DOIAbstract
Biological rejuvenation by partial cell reprogramming is an emerging avenue of research. In this context, regulatable pluripotency gene expression systems are the most widely used at present. We have constructed a regulatable bidirectional adenovector expressing the humanized green fluorescent protein (GFP) and oct4, sox2, klf4, and c-myc genes (known as the Yamanaka genes or OSKM). The OSKM genes are arranged as a bicistronic tandem (hSTEMCCA tandem), which is under the control of a Tet-Off bidirectional promoter that also controls the expression of the gFP gene. Separately, a constitutive cassette expresses the regulatory protein tTA. Vector DNA was transfected in HEK293 Cre cells, which were additionally infected with the helper adenovec...
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