Catalase Stability in Amorphous and Supercooled Media Related to Trehalose- Water- Salt Interactions

Sugars are the most common excipients added to pharmaceutical and biotechnological formulations as protein protectants due to their adequate physicochemical properties, low toxicity, high purity and low cost. However, a second excipient is generally required to extend their protection in supercooled media. The effect of electrolytes is of special interest because of their universal presence in biological systems, their major influence on water structure, and their capability to interact with biomolecules. The purpose of the present work was to analyze the effect of different salts on the stability of catalase in amorphous (glassy and supercooled) trehalose matrices. Trehalose-Mg2+ system was better than trehalose alone to protect the enzyme both during freeze-drying and later storage at low RH (22%). The stabilizing effect observed for certain salts in these systems was not related with an increase of the Tg value. Under conditions at which trehalose crystallizes (43 %RH), salts (especially Mg2+) were detrimental since the enzyme became confined in a salt-concentrated region. Protein denaturation and aggregation were analyzed through differential scanning calorimetry in order to correlate activity changes with physical changes. Trehalose systems without salt and Mg2+-containing systems showed almost no aggregation after denaturation, in agreement with the thermal stability of the enzyme. Thus, the two major parameters related to enzyme stability in freeze-dried non-crystallized systems are: enzyme characteristics (type, quaternary structure) and salt-protein specific interactions.Fil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; ArgentinaFil: Mazzobre, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; ArgentinaFil: Buera, Maria del Pilar. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Químicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Químicos; Argentin