NADPH oxidase and mitochondria are relevant sources of superoxide anion in the oxinflammatory response of macrophages exposed to airborne particulate matter
- Cáceres, Lourdes
- Paz, Mariela Laura
- Garcés, Mariana
- Calabró López, María Valeria
- Magnani, Natalia Daniela
- Martinefski, Manuela
- Martino Adami, Pamela Victoria
- Caltana, Laura Romina
- Tasat, Deborah
- Morelli, Laura
- Tripodi, Valeria Paula
- Valacchi, Giuseppe
- Alvarez, Silvia
- Gonzalez Maglio, Daniel Horacio
- Marchini, Timoteo Oscar
- Evelson, Pablo Andrés
DOIAbstract
Exposure to ambient air particulate matter (PM) is associated with increased cardiorespiratory morbidity and mortality. In this context, alveolar macrophages exhibit proinflammatory and oxidative responses as a result of the clearance of particles, thus contributing to lung injury. However, the mechanisms linking these pathways are not completely clarified. Therefore, the oxinflammation phenomenon was studied in RAW 264.7 macrophages exposed to Residual Oil Fly Ash (ROFA), a PM surrogate rich in transition metals. While cell viability was not compromised under the experimental conditions, a proinflammatory phenotype was observed in cells incubated with ROFA 100 μg/mL, characterized by increased levels of TNF-α and NO production, together wi...