Utilisation of CO₂ from Sodium Bicarbonate to Produce <i>Chlorella vulgaris</i> Biomass in Tubular Photobioreactors for Biofuel Purposes

Microalgae are one of the most promising sources of renewable substrates used for energy purposes. Biomass and components accumulated in their cells can be used to produce a wide range of biofuels, but the profitability of their production is still not at a sufficient level. Significant costs are generated, i.a., during the cultivation of microalgae, and are connected with providing suitable culture conditions. This study aims to evaluate the possibility of using sodium bicarbonate as an inexpensive alternative CO2 source in the culture of Chlorella vulgaris, promoting not only the increase of microalgae biomass production but also lipid accumulation. The study was carried out at technical scale using 100 L photobioreactors. Gravimetric and spectrophotometric methods were used to evaluate biomass growth. Lipid content was determined using a mixture of chloroform and methanol according to the Blight and Dyer method, while the carbon content and CO2 fixation rate were measured according to the Walkley and Black method. In batch culture, even a small addition of bicarbonate resulted in a significant (p ≤ 0.05) increase in the amount of biomass, productivity and optical density compared to non-bicarbonate cultures. At 2.0 g∙L–1, biomass content was 572 ± 4 mg·L−1, the maximum productivity was 7.0 ± 1.0 mg·L–1·d–1, and the optical density was 0.181 ± 0.00. There was also an increase in the lipid content (26 ± 4%) and the carbon content in the biomass (1322 ± 0.062 g∙dw–1), as well as a higher rate of carbon dioxide fixation (0.925 ± 0.073 g·L–1·d–1). The cultivation of microalgae in enlarged scale photobioreactors provides a significant technological challenge. The obtained results can be useful to evaluate the efficiency of biomass and valuable cellular components production in closed systems realized at industrial scale